Saint James City is located on the southernmost tip of Pine Island, in Lee County, Florida, which is part of the Cape Coral-Fort Myers metro region. Saint James City is the most developed part of the island, and is known for its seafood and watering holes, and also for the St. Jude Harbors subdivision, and the Cherry Estates Trailer Park. Notable companies include the Lee County Fishing Guides, who will provide you with all you need for your fishing adventure, Cove Systems Inc. software developers, Aviation Finance Associates, and HIV/STD Testing of Estero, which is actually located in nearby Estero, Florida.