Knowlesville is located in Orleans County, New York. The Erie Canal passes through the town. One major attraction in the area is the Erie Canal Heritage Trail. This is a popular biking and hiking trail in Knowlesville and the surrounding area. Among the biggest companies in Knowlesville are Agway Feed Store and Knowlesville United Methodist Church. There are no schools located in Knowlesville, but area students are able to attend school in the nearby cities of Medina and Albion. One of the best places to visit when you are in Knowlesville is the historic Long Bridge Cemetery.